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National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
Sang Beom Jun 1,2, Verginia Cuzon Carlson 1, Stephen Ikeda 3, David Lovinger 1
1Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, 2Department of Electronics Engineering, Ewha Womans University, 3Section on Transmitter Signaling/Laboratory of Molecular Physiology, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism

This report demonstrates a technique for mechanical isolation of individual viable neurons retaining attached presynaptic boutons. Vibrodissociated neurons have the advantages of rapid production, excellent pharmacological control and improved space-clamp without influence from neighboring cells. This method can be used for imaging of synaptic elements and patch-clamp recording.

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Neuroscience

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording
Youjin Lee *1,2, Daeho Ryu *3, Saeyeong Jeon 3, Yena Lee 1, Yoon Kyung Cho 1, Chang-Hyeon Ji 1,2, Yong-Kweon Kim 3,4, Sang Beom Jun 1,2,5
1Department of Electronic and Electrical Engineering, Ewha Womans University, 2Graduate Program in Smart Factory, Ewha Womans University, 3Department of Electrical and Computer Engineering, Seoul National University, 4Graduate School of Engineering Practice, Seoul National University, 5Department of Brain and Cognitive Sciences, Ewha Womans University

Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording.

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