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The Ohio State University Medical Center

9 ARTICLES PUBLISHED IN JoVE

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Biology

Murine Renal Transplantation Procedure
Jiao-Jing Wang 1, Sara Hockenheimer 2, Alice A. Bickerstaff 1, Gregg A. Hadley 1
1Department of Surgery, The Ohio State University, 2Integrated Biomedical Sciences Graduate Program, The Ohio State University

Renal transplantation in mice is a technically challenging procedure that requires careful post-operative care and treatment for success.

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Medicine

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
Surya C. Gnyawali 1,2, Sashwati Roy 1,2, Jason Driggs 1,2, Savita Khanna 1,2, Thomas Ryan 2,3, Chandan K. Sen 2
1Department of Surgery, The Ohio State University, 2Heart and Lung Research Institute, The Ohio State University, 3Department of Cardiovascular Medicine, The Ohio State University

High frequency Doppler ultrasound is a novel technology for assessing regional myocardial function. This work presents first evidence demonstrating applicability of this versatile imaging platform for the repeated measure of myocardial strain, dp/dt, and mitral regurgitation in the ischemia-reperfused (IR) murine heart.

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Medicine

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
Ronald X. Xu 1, Kun Huang 2, Ruogu Qin 1, Jiwei Huang 1, Jeff S. Xu 1, Liya Ding 2, Urmila S. Gnyawali 3, Gayle M. Gordillo 3, Surya C. Gnyawali 3,4, Chandan K. Sen 3
1Department of Biomedical Engineering, The Ohio State University, 2Department of Biomedical Informatics, The Ohio State University, 3Comprehensive Wound Center, The Ohio State University, 4Department of Surgery, The Ohio State University

A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.

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Medicine

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens
Kaushal Joshi 1, Habibe Demir 1, Ryosuke Yamada 1, Takeshi Miyazaki 1, Abhik Ray-Chaudhury 2, Ichiro Nakano 1
1Department of Neurological Surgery, The Ohio State University Medical Center, 2Department of Pathology, The Ohio State University Medical Center

Here, we established a method for drug efficacy testing with surgical specimens of brain tumors, termed “tumor explant method”. With this method, we can evaluate drug efficacy without breaking the microenvironment of solid tumors. To validate reliability of this method, we describe representative data with our glioma specimen treated with the current first-line chemotherapeutic agent, temozolomide.

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Immunology and Infection

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
Bryan A. Anthony 1, Gregg A. Hadley 1
1Department of Surgery, The Ohio State University Medical Center

Murine bone marrow transplantation is a widely used technique to study immunological mechanisms governing graft-versus-host disease in humans. The ability to monitor T cell trafficking patterns in vivo allows for detailed analysis of the development and perpetuation of T cell responses during graft-versus-host disease.

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Medicine

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
Zhengtao Chu 1,2, Kathleen LaSance 3, Victor Blanco 1, Chang-Hyuk Kwon 5,6, Balveen Kaur 5,6, Malinda Frederick 4, Sherry Thornton 4, Lisa Lemen 3, Xiaoyang Qi 1,2
1Division of Hematology-Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine, 2Division of Human Genetics, University of Cincinnati College of Medicine, 3Department of Radiology, University of Cincinnati College of Medicine, 4Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 5Solid Tumor Biology Program, James Comprehensive Cancer Center, The Ohio State University Medical Center, 6Department of Neurological Surgery, James Comprehensive Cancer Center, The Ohio State University Medical Center

We describe a multi-angle rotational optical imaging (MAROI) system for in vivo quantitation of a fluorescent marker delivered by saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles. Employing mouse models of cancer and arthritis, we demonstrate how the MAROI signal curve analysis can be used for the precise mapping and biological characterization of disease processes.

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Medicine

Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury
Allyson L. Huttinger 1, Debra G. Wheeler 1, Surya Gnyawali 2, David Dornbos III 1, Juliana M. Layzer 3, Nicholas Venetos 1, Spencer Talentino 1, Nicholas J. Musgrave 1, Cheyenne Jones 1, Camille Bratton 1, Matthew E. Joseph 4, Chandan Sen 2, Bruce A. Sullenger 3, Shahid M. Nimjee 1
1Department of Neurological Surgery, Ohio State University, 2Department of Surgery, Ohio State University, 3Department of Surgery, Duke University, 4Department of Internal Medicine, Ohio State University

Here, we present the modifications necessary to a well characterized and commonly used small animal ferric chloride-induced (FeCl3) carotid artery injury model for use in a large animal vascular injury model. The resulting model can be utilized for pre-clinical trial assessment of both prophylactic and thrombolytic pharmacological and mechanical interventions.

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Cancer Research

Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells
Katie A Thies 1, Sarah Steck 1, Sue E Knoblaugh 2, Steven T Sizemore 1
1Department of Radiation Oncology, Arthur G. James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University Medical Center, 2Department of Veterinary Biosciences, Comparative Pathology and Digital Imaging Shared Resource, The Ohio State University

Intravenous injection of cancer cells is often used in metastasis research, but the metastatic tumor burden can be difficult to analyze. Herein, we demonstrate a tail-vein injection model of metastasis and include a novel approach to analyze the resulting metastatic lung tumor burden.

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Neuroscience

Quantification of Cerebral Perfusion using Laser Speckle Imaging and Infarct Volume using MRI in a Pre-clinical Model of Posterior Circulation Stroke
Surya C. Gnyawali 1, Debra G. Wheeler 1, Allyson L. Huttinger 1, Cole Anderson 1, Ian Mandybur 1, Catherine Lee 1, Caitlin Hatten 1, Jenna Boue 2, Matthew Joseph 3, Shahid M. Nimjee 1,2,4
1Department of Neurological Surgery, The Ohio State University Medical Center, 2Department of Anatomy, The Ohio State University Medical Center, 3Department of Internal Medicine, The Ohio State University Medical Center, 4Department of Radiology, The Ohio State University Medical Center

A canine model of LVO stroke was utilized to develop laser speckle imaging to monitor cerebral perfusion in real-time.  Diffusion-weighted MRI was optimized to image infarct volume utilizing a high b-value, enabling ADC and MRA, correlated with DSA at the time of stroke.  Finally, ADC reconstructions correlated with histological findings.

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