University of Duisburg-Essen
2 ARTICLES PUBLISHED IN JoVE
Biology
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Niels Voigt *1,2, Xiao-Bo Zhou *2, Dobromir Dobrev 1,2
1Institute of Pharmacology, University of Duisburg-Essen , 2Division of Experimental Cardiology, University of Heidelberg
We describe the isolation of human atrial myocytes which can be used for intracellular Ca2+ measurements in combination with electrophysiological patch-clamp studies.
Bioengineering
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Fitzwilliam Seibertz 1,2, Martyn Reynolds 3, Niels Voigt 1,2,4
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany, 2DZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Germany, 3Cairn Research Ltd, Faversham, United Kingdom, 4Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Goettingen, Germany
Here we describe optical acquisition and characterization of action potentials from induced pluripotent stem cell derived cardiomyocytes using a high-speed modular photometry system.
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