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A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
Helmar Waiczies 1,2, Martin Guenther 1,2, Julia Skodowski 1,2, Stefano Lepore 1,2, Andreas Pohlmann 2, Thoralf Niendorf 1,2, Sonia Waiczies 1,2
1Experimental and Clinical Research Center, A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine

Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.

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JoVE Core

Cardiac Magnetic Resonance Imaging at 7 Tesla
Daniel Stäb 1,2, Aiman Al Najjar 1, Kieran O'Brien 1,3, Wendy Strugnell 4, Jonathan Richer 3, Jan Rieger 5, Thoralf Niendorf 5, Markus Barth 1
1The Centre for Advanced Imaging, The University of Queensland, Brisbane, Australia, 2Department of Diagnostic and Interventional Radiology, University Clinic Würzburg, Würzburg, Germany, 3Siemens Healthcare Pty Ltd, Brisbane, Australia, 4Richard Slaughter Centre of Excellence in CVMRI, The Prince Charles Hospital, Brisbane, Australia, 5MRI.Tools GmbH, Berlin, Germany

The sensitivity gain inherent to ultrahigh field magnetic resonance holds promise for high spatial resolution imaging of the heart. Here, we describe a protocol customized for functional cardiovascular magnetic resonance (CMR) at 7 Tesla using an advanced multi-channel radio-frequency coil, magnetic field shimming and a triggering concept.

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Medicine

Magnetic Resonance Imaging of Multiple Sclerosis at 7.0 Tesla
Sonia Waiczies *1, Antje Els *1, Joseph Kuchling *2,3,4, Karin Markenroth Bloch 5, Anna Pankowska 6,7, Helmar Waiczies 8, Carl Herrmann 1, Claudia Chien 2,3, Carsten Finke 4,9, Friedemann Paul 2,3,4, Thoralf Niendorf 1,2,8
1Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 2Experimental and Clinical Research Center, Max Delbrueck Center for Molecular Medicine and Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3NeuroCure Clinical Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 4Department of Neurology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 5The Swedish National 7T Facility, Lund University Bioimaging Center, Lund University, 6Department of Radiography, Medical University of Lublin, 7ECOTECH-COMPLEX, Maria Curie-Skłodowska University, 8MRI.TOOLS GmbH, 9Berlin School of Mind and Brain, Humboldt-Universität zu Berlin

Here, we present a protocol to acquire magnetic resonance (MR) images of multiple sclerosis (MS) patient brains at 7.0 Tesla. The protocol includes preparation of the setup including the radio-frequency coils, standardized interview procedures with MS patients, subject positioning in the MR scanner and MR data acquisition.

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