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University of Ulm

5 ARTICLES PUBLISHED IN JoVE

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Medicine

Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases
Hans-Peter Müller 1, Jan Kassubek 1
1Department of Neurology, University of Ulm

Diffusion tensor imaging (DTI) basically serves as an MRI-based tool to identify in vivo the microstructure of the brain and pathological processes due to neurological disorders within the cerebral white matter. DTI-based analyses allow for application to brain diseases both at the group level and in single subject data.

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Developmental Biology

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Sathish Venkataramanappa 1, Ruth Simon 1, Stefan Britsch 1
1Institute of Molecular and Cellular Anatomy, University of Ulm

Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.

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Immunology and Infection

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Valerio Rossini 1, Katarina Radulovic 2, Christian U. Riedel 3, Jan Hendrik Niess 2
1APC Microbiome Institute, University College Cork, 2Division of Gastroenterology and Hepatology, University Hospital Basel, 3Institute of Microbiology and Biotechnology, University of Ulm

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

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Behavior

Eye-Tracking Control to Assess Cognitive Functions in Patients with Amyotrophic Lateral Sclerosis
Jürgen Keller 1, Martin Gorges 1, Helena E. A. Aho-Özhan 1, Ingo Uttner 1, Erich Schneider 2, Jan Kassubek 1, Elmar H. Pinkhardt 1, Albert C. Ludolph 1, Dorothée Lulé 1
1Department of Neurology, Ulm University, 2Institute of Medical Technology, Brandenburg University of Technology Cottbus-Senftenberg

Cognitive deficits are common in about one third of patients with amyotrophic lateral sclerosis, a neurological condition leading to progressive impairments in speech and movement abilities. To conduct cognitive tests in patients unable to speak or write a reliable and easy to administer eye-tracking paradigm was developed.

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Neuroscience

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
Christoph Wiegreffe 1, Svenja Feldmann 1, Simeon Gaessler 1, Stefan Britsch 1
1Institute of Molecular and Cellular Anatomy, Ulm University

This protocol provides instructions for direct observation of radially migrating cortical neurons. In utero electroporation, organotypic slice culture, and time-lapse confocal imaging are combined to directly and dynamically study the effects of overexpression or downregulation of genes of interest in migrating neurons and to analyze their differentiation during development.

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