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IRCCS - Istituto di Ricerche Farmacologiche Mario Negri

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
Carlo Perego *1, Stefano Fumagalli *1, Maria-Grazia De Simoni 1
1Department of Neuroscience, IRCCS - Istituto di Ricerche Farmacologiche Mario Negri

A way to gain new insights into the complexity of the brain inflammatory response is presented. We describe immunofluorescence-based protocols followed by three-dimensional confocal analysis to investigate the pattern of co-expression of microglia/macrophage phenotype markers in a mouse model of focal ischemia.

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