Montreal Heart Institute

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Permeabilization of Adhered Cells Using an Inert Gas Jet

Scott Cooper¹, Paul Jonak¹, Guillaume Chouinard-Pelletier¹, Sylvain Coulombe¹, Elizabeth Jones¹, Richard L. Leask^1,2

¹Chemical Engineering, McGill University, ²Montreal Heart Institute

This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.

Advancing the Assessment of Cardiac Injury and Function

Ienglam Lei¹, Pierre-Emmanuel Noly², Paul C. Tang¹

¹Department of Cardiac Surgery, University of Michigan, Ann Arbor, ²Department of Cardiac Surgery, Montreal Heart Institute

Advancing the Assessment of Cardiac Injury and Function

Medicine

Rat Model of Right-Sided Cardiac Remodeling and Arrhythmia Using Pulmonary Artery Banding

Charles-Alexandre LeBlanc¹, Marie-Ève Higgins¹, Martin G. Sirois^1,2, Jean-François Tanguay^1,3, Jean-Claude Tardif^1,3, Roddy Hiram^1,3

¹Montreal Heart Institute, ²Department of Pharmacology and Physiology, Université de Montréal, ³Department of Medicine, Faculty of Medicine, Université de Montréal

Right heart failure (RHF) is characterized by right-sided cardiac dilation and hypertrophy, leading to ventricular and atrial malfunction. Cardiopulmonary conditions associated with RHF are accompanied by an increased risk for cardiac arrhythmias. This article describes a standardized model of pulmonary artery banding-induced RHF associated with enhanced ventricular and atrial arrhythmogenesis.