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London School of Hygiene & Tropical Medicine

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
Neveda Naz 1, Dominic C. Mills 1, Brendan W. Wren 1, Nick Dorrell 1
1Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine

Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.

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