Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM)

6 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

Filippos Porichis^*1, Meghan G. Hart^*1, Jennifer Zupkosky¹, Lucie Barblu^1,2, Daniel E. Kaufmann^1,2

¹The Ragon Institute of MGH, MIT and Harvard, ²Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM)

We developed an in vitro assay to investigate the role of immunoregulatory pathways in the regulation of cytokine secretion by HIV-specific CD4 T cells.

Medicine

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

Yoko A. Ito^*1, Nicolas Belforte^*1, Jorge L. Cueva Vargas¹, Adriana Di Polo¹

¹Department of Neuroscience, CRCHUM and University of Montreal

Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.

Medicine

Pre-clinical Model of Cardiac Donation after Circulatory Death

Henry Aceros^*1, Leyla Joulali^*2, Mélanie Borie¹, Roberto Vanin Pinto Ribeiro³, Mitesh Vallabh Badiwala³, Shant Der Sarkissian^1,4, Nicolas Noiseux^1,4

¹Centre de recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), ²Deparment of pharmacology and physiology, Faculty of Medicine, Université de Montréal, ³Division of Cardiovascular Surgery, Toronto General Hospital, University Health Network, ⁴Department of surgery, Faculty of Medicine, Université de Montréal

This protocol shows a simple and flexible approach for the evaluation of new conditioning agents or strategies to increase the feasibility of cardiac donation after circulatory death.

Genetics

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Francis Migneault^1,2,3, Frédéric Gagnon^2,3, Emmanuelle Brochiero^1,3, Yves Berthiaume^2,3, André Dagenais^2,3

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Institut de Recherches Cliniques de Montréal (IRCM), ³Département de médecine, Faculté de médecine, Université de Montréal

Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.

Immunology and Infection

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Manuel Flores Molina^1,2, Thomas Fabre^1,2, Aurélie Cleret-Buhot¹, Geneviève Soucy^1,3, Liliane Meunier^1,4, Mohamed N. Abdelnabi^1,2, Nicolas Belforte^1,5, Simon Turcotte^1,4,6, Naglaa H. Shoukry^1,4,7

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, ³Département de Pathologie et Biologie Cellulaire, Faculté de Médecine, Université de Montréal, ⁴Institut du Cancer de Montréal, ⁵Département de Neurosciences, Faculté de Médecine, Université de Montréal, ⁶Département de Chirurgie, Faculté de Médecine, Université de Montréal, ⁷Département de Médecine, Faculté de médecine, Université de Montréal

Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.

Medicine

Large-Animal Model of Donation after Circulatory Death and Normothermic Regional Perfusion for Cardiac Assessment

Khalil Khalil^*1,2, Roberto V. P. Ribeiro^*3, Julgans S. Alvarez^4,5, Mitesh V. Badiwala^4,5, Shant Der Sarkissian^1,2, Nicolas Noiseux^1,2

¹Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), ²Department of Surgery, Faculty of Medicine, Université de Montréal, ³Division of Cardiac Surgery, Dalhousie University, ⁴Division of Cardiovascular Surgery, Peter Munk Cardiac Centre, Toronto General Hospital, ⁵Department of Surgery, University of Toronto

The protocol describes a large-animal (porcine) model of donation after circulatory death, followed by thoracoabdominal normothermic regional perfusion that closely simulates the clinical scenario in heart transplantation, and has the potential to facilitate therapeutic studies and strategies.