University of Naples "Federico II"

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Bone Marrow-derived Macrophage Production

Virginie Trouplin¹, Nicolas Boucherit¹, Laurent Gorvel¹, Filippo Conti¹, Giovanna Mottola^1,2, Eric Ghigo¹

¹CNRS UMR 7278, IRD198, INSERM U1095, Aix-Marseille Université, ²Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II"

Macrophages have long been recognized as a critical component of the innate and adaptive immune responses. The recent explosion of knowledge pertaining to evolutionary, genetic, and biochemical aspects of the interaction between macrophages and microbes has renewed scientific attention to macrophages. This article describes a method to differentiate macrophages from mouse bone marrow.

Immunology and Infection

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens

Rachele Isticato¹, Ezio Ricca¹, Loredana Baccigalupi²

¹Department of Biology, Federico II University, ²Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II"

This protocol focuses on the use of bacterial spores as a "live" nanobiotechnological tool to adsorb heterologous molecules with various biological activities. The methods to measure the efficiency of adsorption are also shown.