A method is described for analyzing protein degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extract and its adaptation for high-throughput screening for small molecule modulators of protein degradation.

reconstitution-of-catenin-degradation-in-xenopus-egg-extract

Reconstitution Of &#946;-catenin Degradation In Xenopus Egg Extract

Xenopus laevis egg extract is a well-characterized, robust system for studying the biochemistry of diverse cellular processes. Xenopus egg extract has ...

A method of visualizing and quantifying the 3-dimensional structure of mouse hepatic portal vein or intrahepatic bile duct is described. This resin cast technique can also be applied to other ductal or vascular systems and allows for in situ visualization or quantification of a system's intact communicating architecture.

3-dimensional-resin-casting-imaging-mouse-portal-vein-or-intrahepatic

Institution - Cincinnati Children's Hospital Medical Center

Articles Published in JoVE

Institution - Cincinnati Children&#39;s Hospital Medical Center

Articles Published in JoVE

Institution - Cincinnati Children's Hospital Medical Center