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Ludwig Maximilians University Munich

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
Marisa Karow 1, Christian Schichor 2, Ruth Beckervordersandforth 3, Benedikt Berninger 1,4
1Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic, Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience, Johannes Gutenberg University Mainz

Targeting brain-resident cells for direct lineage-reprogramming offers new perspectives for brain repair. Here we describe a protocol of how to prepare cultures enriched for brain-resident pericytes from the adult human cerebral cortex and convert these into induced neurons by retrovirus-mediated expression of the transcription factors Sox2 and Ascl1.

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Medicine

Development and Evaluation of 3D-Printed Cardiovascular Phantoms for Interventional Planning and Training
Maximilian Grab 1,2, Carina Hopfner 3, Alena Gesenhues 4, Fabian König 1,2, Nikolaus A. Haas 3, Christian Hagl 1, Adrian Curta 4, Nikolaus Thierfelder 1
1Department of Cardiac Surgery, Ludwig Maximilian University Munich, 2Chair of Medical Materials and Implants, Technical University of Munich, 3Department Pediatric Cardiology and Pediatric Intensive Care, Ludwig Maximilian University Munich, 4Department of Radiology, Ludwig Maximilian University Munich

Here we present development of a mock circulation setup for multimodal therapy evaluation, pre-interventional planning, and physician-training on cardiovascular anatomies. With the application of patient-specific tomographic scans, this setup is ideal for therapeutic approaches, training, and education in individualized medicine.

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Medicine

Combining 3D-Printing and Electrospinning to Manufacture Biomimetic Heart Valve Leaflets
Benedikt Freystetter 1, Maximilian Grab 1,2, Linda Grefen 1, Lara Bischof 1, Lorenz Isert 3, Petra Mela 2, Deon Bezuidenhout 4, Christian Hagl 1,5, Nikolaus Thierfelder 1
1Department of Cardiac Surgery, Ludwig Maximilians University Munich, 2Chair of Medical Materials and Implants, Technical University Munich, 3Faculty for Chemistry and Pharmacy, Ludwig Maximilians University Munich, 4Cardiovascular Research Unit, University of Cape Town, 5DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance

The presented method offers an innovative way for engineering biomimetic fiber structures in three-dimensional (3D) scaffolds (e.g., heart valve leaflets). 3D-printed, conductive geometries were used to determine shape and dimensions. Fiber orientation and characteristics were individually adjustable for each layer. Multiple samples could be manufactured in one setup.

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