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College of William & Mary

1 ARTICLES PUBLISHED IN JoVE

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Biology

Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
Ashley Fidler *1, Lauren Boulay *1, Matthew Wawersik 1
1Department of Biology, College of William & Mary

Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.

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