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University of California at San Francisco

4 ARTICLES PUBLISHED IN JoVE

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Biology

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras
Jennifer L. Fish 1, Richard A. Schneider 1
1Department of Orthopaedic Surgery, University of California at San Francisco

This article describes a method to generate chimeric embryos that are designed to test the species-specific contributions of neural crest and/or other tissues to craniofacial development.

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Developmental Biology

Methods for the Study of Regeneration in Stentor
Athena Lin 1, Tatyana Makushok 1, Ulises Diaz 1, Wallace F. Marshall 1
1Department of Biochemistry and Biophysics, University of California San Francisco

The giant ciliate, Stentor coeruleus, is an excellent system to study regeneration and wound healing. We present procedures for establishing Stentor cell cultures from single cells or cell fragments, inducing regeneration by cutting cells, chemically inducing the regeneration of membranellar band and oral apparatus, imaging, and analysis of cell regeneration.

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Developmental Biology

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Natasja G. J. Costermans 1,2, Enrico M. Daldello 1,2,3, Ria J. Marathe 1,2, Marco Conti 1,2
1Center for Reproductive Sciences, University of California at San Francisco, 2Department of Obstetrics Gynecology and Reproductive Sciences, University of California at San Francisco, 3Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS, Sorbonne Université

This protocol describes a reporter assay to study the regulation of mRNA translation in single oocytes during in vitro maturation.

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Biology

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking
Janet Y. Sheung 1, Megan Otsuka 2, Gabriella Seifert 3, Athena Lin 4, Wallace F. Marshall 4
1W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, 2W. M. Keck Science Department, Pitzer College, 3W. M. Keck Science Department, Scripps College, 4Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.

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