U. S. Dept. of Veterans Affairs

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability

Kathryn Patricia Haley¹, Eric Joshua Blanz¹, Jennifer Angeline Gaddy^1,2

¹Department of Medicine - Division of Infectious Diseases, Vanderbilt University School of Medicine, ²Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs

Here we describe a method to visualize the oncogenic bacterial organelle known as the Cag Type IV Secretion System (Cag-T4SS). We find that the Cag-T4SS is differentially produced on the surface of H. pylori in response to varying conditions of iron availability.

Biochemistry

Expression, Purification, and Antimicrobial Activity of S100A12

Emmanuel Jackson¹, Saffron Little¹, Dana S. Franklin¹, Jennifer A. Gaddy^2,3, Steven M. Damo^1,4

¹Department of Life and Physical Sciences, Fisk University, ²Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs, ³Department of Medicine - Division of Infectious Diseases, Vanderbilt University Medical School, ⁴Department of Biochemistry, Vanderbilt University

Here, we present a method to express and purify S100A12 (calgranulin C). We describe a protocol to measure its antimicrobial activity against the human pathogen H. pylori.