Proteomic Profiling of Macrophages by 2D ElectrophoresisMarion Bouvet 1, Annie Turkieh 1, Adelina E. Acosta-Martin 1, Maggy Chwastyniak 1, Olivia Beseme 1, Philippe Amouyel 1, Florence Pinet 1
1Inserm UMR744, University Lille Nord de France
Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.
