Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by ElectroporationKhujista D. Haque 1, Atul K. Pandey 1, Matthew W. Kelley 2, Chandrakala Puligilla 1
1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, College of Medicine, 2Laboratory of Cochlear Development, NIDCD, NIH
We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.