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Friedrich Miescher Laboratory of the Max Planck Society

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
Katherine W. Rogers 1, Alexander Bläßle 2, Alexander F. Schier 1, Patrick Müller 2
1Department of Molecular and Cellular Biology, Harvard University, 2Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society

Protein levels in cells and tissues are often tightly regulated by the balance of protein production and clearance. Using Fluorescence Decay After Photoconversion (FDAP), the clearance kinetics of proteins can be experimentally measured in vivo.

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Developmental Biology

A Simple and Effective Transplantation Device for Zebrafish Embryos
Gary H. Soh 1, Anna C. Kögler 1, Patrick Müller 1,2
1Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society, 2University of Konstanz

Embryological manipulations such as extirpation and transplantation of cells are important tools to study early development. This protocol describes a simple and effective transplantation device to perform these manipulations in zebrafish embryos.

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