Contrast Imaging in Mouse Embryos Using High-frequency UltrasoundJanet M. Denbeigh 1,2, Brian A. Nixon 1,2, Mira C. Puri 1,2,3, F. Stuart Foster 1,2
1Department of Medical Biophysics, University of Toronto, 2Sunnybrook Research Institute, 3Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto
Here, we present a protocol to inject ultrasound microbubble contrast agents into living, isolated late-gestation stage murine embryos. This method enables the study of perfusion parameters and of vascular molecular markers within the embryo using contrast-enhanced high-frequency ultrasound imaging.