CHUV, Université de Montréal, St-Hyacinthe, QC

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Patrick Burns¹, Hai Lun Liu², Shikha Kuthiala², Gilles Fecteau¹, André Desrochers¹, Lucien Daniel Durosier², Mingju Cao², Martin G. Frasch^2,3,4

¹Département de sciences cliniques, CHUV, Université de Montréal, St-Hyacinthe, QC, ²Département d'obstetriques et de gynécologie, CHU Ste-Justine Research Centre, Université de Montréal, ³Département de neurosciences, CHU Ste-Justine Centre de recherche, Université de Montréal, ⁴Centre de recherche en reproduction animale (CRRA), Université de Montréal, St-Hyacinthe, QC

The chronically instrumented non-anesthetized fetal sheep model is used to study human fetal development in health and disease, because it permits surgical placement and maintenance of catheters and electrodes, repetitive blood sampling, substance injection, recording of bioelectrical activity, and in vivo imaging. We describe the procedures required to establish this model.

Medicine

A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence

Eric Parent¹, Patrick Burns², André Desrochers¹, Martine Boulianne¹

¹Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, ²University of Bristol

Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.