University of Newcastle, Callaghan, New South Wales, Australia

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Sergey Kurdyukov¹, Youhong Song¹, Terence W-Y Tiew¹, Xin-Ding Wang¹, Kim E. Nolan¹, Ray J. Rose¹

¹School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia

The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.