University of Michigan Health System

3 ARTICLES PUBLISHED IN JoVE

Medicine

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test

Sujay S. Galen¹, Darren R. Guffey², Jared W. Coburn³, Moh H. Malek¹

¹Physical Therapy Program and Integrative Physiology of Exercise Laboratory, Department of Health Care Sciences, Wayne State University, ²Sports Medicine and Physical Therapy, MEDSPORT, University of Michigan Health System, ³Department of Kinesiology, California State University, Fullerton

This protocol describes the electromyographic fatigue threshold which demarcates between nonfatiguing and fatiguing exercise workloads. This information could be used to develop a more individualized training program.

Medicine

Comparing the Effects of Electronic Cigarette Vapor and Cigarette Smoke in a Novel In Vivo Exposure System

Anthony N. Hage¹, Will Krause¹, Angela Mathues¹, Luke Krasner², Seth Kasten¹, Jonathan L. Eliason^1,3, Abhijit Ghosh¹

¹Jobst Vascular Research Laboratory, University of Michigan Medical School, ²Department of Engineering, Purdue University, ³Department of Surgery, Section of Vascular Surgery, University of Michigan Health System

This protocol describes a method for exposing rodents to electronic cigarette vapor (E-vapor) and cigarette smoke. Exposure chambers are constructed by modifying anesthesia chambers with an automated pumping system that delivers E-vapor or cigarette smoke to rodents. This system can easily be modified to accommodate many experimental endpoints.

JoVE Core

Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands

Isabella Finco¹, Gary D. Hammer^1,2,3,4,5

¹Department of Internal Medicine (Division of Metabolism, Endocrinology, and Diabetes), University of Michigan Health System, ²Department of Cell and Developmental Biology, University of Michigan Health System, ³Department of Molecular and Integrative Physiology, University of Michigan Health System, ⁴Endocrine Oncology Program, University of Michigan Health System, ⁵Comprehensive Cancer Center, University of Michigan Health System

Here we present a method to isolate adrenal glands from mice, fix the tissues, section them, and perform immunofluorescence staining.