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Bundeswehr Institute of Pharmacology and Toxicology

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
Dirk Steinritz 1,2, Annette Schmidt 1,3, Frank Balszuweit 1, Horst Thiermann 1, Marwa Ibrahim 3, Birgit Bölck 3, Wilhelm Bloch 3
1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-Universität München, 3Department of Molecular and Cellular Sports Medicine, German Sports University Cologne

Investigation of early endothelial cell (EEC) migration is important to understand the pathophysiology of certain illnesses and to potentially identify novel strategies for therapeutic intervention. The following protocol describes techniques to assess cell migration that have been adapted for the investigation of EEC.

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Medicine

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
Amelie Tsoutsoulopoulos 1, Katrin Gohlsch 2, Niklas Möhle 3, Andreas Breit 2, Sebastian Hoffmann 5, Olaf Krischenowski 3,4, Harald Mückter 2, Thomas Gudermann 2, Horst Thiermann 1, Michaela Aufderheide 3,4, Dirk Steinritz 1,2
1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology, University of Munich, 3Cultex Laboratories GmbH, 4Cultex Technologies GmbH (formerly Cultex Laboratories GmbH), 5seh consulting + services

We present a robust, transferable and predictive in vitro exposure system for the screening and monitoring of airborne particles concerning their acute pulmonary cytotoxicity by exposing cultivated human lung cells at the air-liquid interface (ALI).

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