Leidos Biomedical Research, Inc.

2 ARTICLES PUBLISHED IN JoVE

Biology

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Anna Coffey^1,2, Michael D. Johnson³, Deborah L. Berry³

¹Center for Advanced Preclinical Research (CAPR), Frederick National Laboratory for Cancer Research, ²Leidos Biomedical Research, Inc., ³Department of Oncology, Georgetown University

The purpose of the Specimen Orientation Tag (SpOT) is to function as an orientation tool to aid in individual tissue identification in multi-tissue paraffin blocks. These protocols demonstrate how it is constructed easily from common, low-cost histology materials and serves as a reliable visual marker in paraffin blocks and sections.

Cancer Research

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Robert Y. S. Cheng¹, Nimit L. Patel², Timothy Back¹, Debashree Basudhar¹, Veena Somasundaram¹, Joseph D. Kalen², David A. Wink¹, Lisa A. Ridnour¹

¹Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick, ²Small Animal Imaging Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc.

This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.