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Seoul National University Hospital

8 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
Sung Wook Park 1,2, Jin Hyoung Kim 2, Woo Jin Park 3, Jeong Hun Kim 1,2,4
1Department of Biomedical Sciences, Seoul National University College of Medicine, 2FARB Laboratory, Biomedical Research Institute, Seoul National University Hospital, 3College of Life Sciences, Gwangju Institute of Science and Technology, 4Department of Ophthalmology, Seoul National University College of Medicine

Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.

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Medicine

Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs
Seong Joon Ahn 1,2,3, Hye Kyoung Hong 1, Young Mi Na 1, Sang Jun Park 1,2, Jeeyun Ahn 1,2,4, Jaeseong Oh 5, Jae Yong Chung 6, Kyu Hyung Park 1,2, Se Joon Woo 1,2
1Department of Ophthalmology, Seoul National University Bundang Hospital, 2Department of Ophthalmology, College of Medicine, Seoul National University, 3Department of Ophthalmology, Hanyang University Hospital, 4Department of Ophthalmology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, 5Department of Clinical Pharmacology, Seoul National University Hospital, 6Department of Clinical Pharmacology, Seoul National University Bundang Hospital

Rabbits are widely used to study the pharmacokinetics of intraocular drugs. We describe a method for conducting pharmacokinetic studies of intraocular drugs using rabbit eyes.

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JoVE Core

Enhancement of the Initial Growth Rate of Agricultural Plants by Using Static Magnetic Fields
Seung C. Kim 1, Alex Mason 2, Wooseok Im 3
1College of Medicine, Yonsei University, 2Simon Fraser University, 3Biomedical Research Institute, Seoul National University Hospital

The goal of this protocol is to demonstrate the acceleration of the initial growth rate of plants by applying static magnetic fields with no external energy.

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Behavior

Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain
Jaewon Beom 1,2, Jung Chan Lee 2,3,4, Jin Chul Paeng 5, Tai Ryoon Han 6, Moon Suk Bang 7, Byung-Mo Oh 7
1Department of Rehabilitation Medicine, Chungnam National University Hospital, Daejeon, 2Department of Biomedical Engineering, Seoul National University College of Medicine, 3Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, 4Department of Biomedical Engineering, Seoul National University Hospital, 5Department of Nuclear Medicine, Seoul National University Hospital, Seoul National University College of Medicine, 6Department of Rehabilitation Medicine, Gangwon Do Rehabilitation Hospital, 7Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul National University College of Medicine

We applied repetitive transcranial magnetic stimulation (rTMS) to the unilateral hemisphere of rat brain, by placing a 25-mm figure-8 coil 1 cm lateral to the vertex on the biauricular line and angulating the coil by 45°. An in-house water cooling system was used for rTMS for more than 20 min.

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Bioengineering

Robotic Mirror Therapy System for Functional Recovery of Hemiplegic Arms
Jaewon Beom *1,2, Sukgyu Koh *3, Hyung Seok Nam 1, Wonshik Kim 3, Yoonjae Kim 3, Han Gil Seo 4, Byung-Mo Oh 4,5, Sun Gun Chung 4,5, Sungwan Kim 1,6
1Department of Biomedical Engineering, Seoul National University College of Medicine, 2Department of Rehabilitation Medicine, Chungnam National University Hospital, 3Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, 4Department of Rehabilitation Medicine, Seoul National University Hospital, 5Seoul National University College of Medicine, 6Institute of Medical and Biological Engineering, Seoul National University

We developed a real-time mirror robot system for functional recovery of hemiplegic arms using automatic control technology, conducted a clinical study on healthy subjects, and determined tasks through feedback from rehabilitation doctors. This simple mirror robot can be applied effectively to occupational therapy in stroke patients with a hemiplegic arm.

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Medicine

Surgical Labyrinthectomy of the Rat to Study the Vestibular System
Mun Young Chang 1, Moo Kyun Park 2, So Hyeon Park 2, Myung-Whan Suh 2, Jun Ho Lee 2, Seung Ha Oh 2
1Department of Otolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, 2Department of Otolaryngology-Head and Neck Surgery, Seoul National University Hospital

This protocol describes the surgical labyrinthectomy of a rat, which is a useful method for studying the vestibular system.

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Bioengineering

Design and Synthesis of a Reconfigurable DNA Accordion Rack
Yeongjae Choi *1, Hansol Choi *1, Amos C. Lee *2, Sunghoon Kwon 1,2,3,4
1Department of Electrical and Computer Engineering, Seoul National University, 2Interdisciplinary Program for Bioengineering, Seoul National University, 3Institute of Entrepreneurial Bio Convergence, Seoul National University, 4Seoul National University Hospital Biomedical Research Institute, Seoul National University Hospital

We describe the detailed protocol for design, simulation, wet-lab experiments, and analysis for a reconfigurable DNA accordion rack of 6 by 6 meshes.

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Bioengineering

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
Daehan Kim 1, Gi-Hun Lee 1, Jiheum Park 2, Jung Chan Lee 2, Joong Yull Park 1
1Department of Mechanical Engineering, Graduate School, Chung-Ang University, 2Department of Biomedical Engineering, College of Medicine, Seoul National University

We present a motor-powered centrifugal microfluidic device that can cultivate cell spheroids. Using this device, spheroids of single or multiple cell types could be easily cocultured under high gravity conditions.

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