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Imperial College

4 ARTICLES PUBLISHED IN JoVE

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Medicine

A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine
Mitsuhiro Yoneda 1, Alfredo A. Molinolo 2, Jerrold M. Ward 3, Shioko Kimura 1, Robert A. Goodlad 4
1National Cancer Institute, Laboratory of Metabolism, National Institutes of Health, 2National Institute of Dental and Craniofacial Research, Oral and Pharyngeal Cancer Branch, National Institutes of Health, 3Global VetPathology, 4Centre for Pathology, Hammersmith Hospital, Imperial College

The use of a simple device to cut and ‘roll’ mouse intestines to rapidly prepare whole mount preparations is described.

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Medicine

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
Victoria L. Patterson *1, Brian S. Thompson *1, Catherine Cherry *1, Shao-bin Wang 2, Bo Chen 2, Josephine Hoh 1,2
1Department of Environmental Health Sciences, Yale University School of Medicine, 2Department of Ophthalmology, Yale University School of Medicine

A reverse-genetics approach to understanding gene families associated with human disease is presented, using mouse as a model system, and the subsequent mouse phenotyping schedule is described. Because mice defective in a gene of interest, HtrA2, manifested Parkinsonian symptoms, the phenotyping regimen is focused on identifying neurological defects.

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Neuroscience

Concurrent Recording of Co-localized Electroencephalography and Local Field Potential in Rodent
Sungmin Kang 1, Michael Bruyns-Haylett 2, Yurie Hayashi 1, Ying Zheng 1
1School of Biological Sciences, Whiteknights, University of Reading, 2Department of Bioengineering, Imperial College

This protocol describes a simple method for concurrent recording of co-localized electroencephalography (EEG) and multi-laminar local field potential in an anesthetized rat. A burr hole drilled in the skull for the insertion of a microelectrode is shown to produce negligible distortion of the EEG signal.

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Immunology and Infection

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
Ana Maria Ortega-Prieto 1, Jessica Katy Skelton 1, Catherine Cherry 1, Marco Antonio Briones-Orta 1, Charlotte Alexandra Hateley 1, Marcus Dorner 1
1Section of Virology, Department of Medicine, Imperial College

The goal of this protocol is to provide a step-by-step guide to perform 3-D "liver-on-a-chip" infection experiments with the hepatitis B virus.

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