MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Anne-Cécile Boulay^1,2,3, Bruno Saubaméa⁴, Xavier Declèves⁴, Martine Cohen-Salmon^1,2,3

¹Collège de France, Center for Interdisciplinary Research in Biology (CIRB), Centre National de la Recherche Scientifique CNRS, Unité Mixte de Recherche 7241, Institut National de la Santé et de la Recherche Médicale, ²Centre Interdisciplinaire de Recherche en Biologie, ³MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University, ⁴Université Paris Descartes, Faculté de Pharmacie, Université Paris Diderot

We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.