Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine TonsilsFarzaneh Assadian 1, Karl Sandström 2, Göran Laurell 2, Catharina Svensson 1, Göran Akusjärvi 1, Tanel Punga 1
1Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, 2Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery, Akademiska sjukhuset
Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.