Université Pierre and Marie Curie

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Miguel A. Lopez-Ramirez^*1,2, Charles-Félix Calvo^*3, Emma Ristori¹, Jean-Léon Thomas^1,3, Stefania Nicoli¹

¹Yale Cardiovascular Research Center, Internal Medicine, Yale University, ²Department of Medicine, University of California, San Diego, ³APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie

Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.