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National Heart Centre Singapore

1 ARTICLES PUBLISHED IN JoVE

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Engineering

In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart
Sindhu Ramesh *1, Anamika Singh *1, Dasan M. Cibi 1, Derek J. Hausenloy 1,2,3, Manvendra K. Singh 1,2
1Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School, 2National Heart Research Institute Singapore, National Heart Centre Singapore, 3The Hatter Cardiovascular Insititute, University College London

The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.

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