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National Sun Yat-sen University

6 ARTICLES PUBLISHED IN JoVE

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Environment

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
Kuo-Tung Jiann *1, Liang-Saw Wen *2,3, Peter H. Santschi 4
1Department of Oceanography, National Sun Yat-sen University, 2Institute of Oceanography, National Taiwan University, 3Taiwan Ocean Research Institute, National Research Laboratories, 4Department of Oceanography and Marine Sciences, Texas A&M University at Galveston

Special care using "clean techniques" is required to properly collect and process water samples for trace metal studies in aquatic environments. A protocol for sampling, processing, and analytical procedures with the aim of obtaining reliable environmental monitoring data and results with high sensitivity for detailed trace metal studies is presented.

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Environment

Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
Wen-Ta Li 1, Bang-Yeh Liou 1, Wei-Cheng Yang 2, Meng-Hsien Chen 3, Hui-Wen Chang 1,2, Hue-Ying Chiou 4, Victor Fei Pang 1,2, Chian-Ren Jeng 1,2
1Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, 2School of Veterinary Medicine, National Taiwan University, 3Department of Oceanography and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, 4Graduate Institute of Veterinary Pathobiology, National Chung Hsing University

A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.

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Immunology and Infection

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
Liang-Chun Wang 1, Jacob Wagner 2, Annabelle Capino 2, Elizabeth Nesbit 2, Wenxia Song 2, Daniel C. Stein 2
1Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, 2Department of Cell Biology and Molecular Genetics, University of Maryland

A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms.

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Medicine

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats
Hsin-Ting Lin 1,2, Yi-Lin Shiou 1, Wun-Jyun Jhuang 1, Hsiang-Chun Lee 1,3,4,5,6
1Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 2Hematology and Oncology, China Medical University Hospital, 3Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 4Center for Lipid Biosciences, Kaohsiung Medical University Hospital, 5Lipid Science and Aging Research Center, Kaohsiung Medical University, 6Institute/Center of Medical Science and Technology, National Sun Yat-sen University

Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.

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Biology

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Ke-Rui Chou 1, HsinYuan Tsai 1,2
1Department of Marine Biotechnology and Resources, National Sun Yat-sen University, 2Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University

The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously.

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Biology

Effective Techniques for the Feeding and Ex Situ Culture of a Brooding Scleractinian Coral, Pocillopora acuta
Kwok-Wai Lam 1, Crystal J. McRae 1, Zhao-Ting Liu 2, Xuan-Ci Zhang 1, Tung-Yung Fan 1,2
1Department of Planning and Research, National Museum of Marine Biology & Aquarium, 2Department of Marine Biotechnology and Resources, National Sun Yat-sen University

Climate change is impacting coral reef ecosystems globally. Corals sourced from ex situ aquaculture systems can help support restoration and research efforts. Herein, feeding and coral culture techniques that can be used to promote the long-term maintenance of brooding scleractinian corals ex situ are outlined.

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