Stony Brook University School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Biology

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Agnieszka B. Bialkowska^*1, Amr M. Ghaleb^*1, Mandayam O. Nandan¹, Vincent W. Yang^1,2

¹Department of Medicine, Stony Brook University School of Medicine, ²Department of Physiology & Biophysics, Stony Brook University School of Medicine

Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.

Medicine

Bedside Ultrasound for Guiding Fluid Removal in Patients with Pulmonary Edema: The Reverse-FALLS Protocol

Danielle Nichole O’Hara¹, Vikram Chabra², Sahar Ahmad²

¹Stony Brook University School of Medicine, ²Stony Brook University Medical Center, Stony Brook University School of Medicine

We describe an imaging protocol that enables the clinician to visualize, in real-time, the patient's intravascular and extravascular space volumes and set diuresis and fluid removal parameters accordingly for the safe and efficient treatment of pulmonary edema.

Biology

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Emilia J. Orzechowska-Licari¹, Joseph F. LaComb¹, Michael Giarrizzo¹, Vincent W. Yang^1,2, Agnieszka B. Bialkowska¹

¹Department of Medicine, Renaissance School of Medicine at Stony Brook University, ²Department of Physiology and Biophysics, Renaissance School of Medicine at Stony Brook University

The gastrointestinal tract is one of the most sensitive organs to injury upon radiotherapeutic cancer treatments. It is simultaneously an organ system with one of the highest regenerative capacities following such insults. The presented protocol describes an efficient method to study the regenerative capacity of the intestinal epithelium.