Universidad de la República (UDELAR)

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

Mercedes Rodriguez-Teja¹, Claudia Breit², Mitchell Clarke³, Kamil Talar³, Kai Wang³, Mohammad A. Mohammad³, Sage Pickwell³, Guillermina Etchandy¹, Graeme J. Stasiuk³, Justin Sturge³

¹Departamento de Genética, Facultad de Medicina, Universidad de la República (UDELAR), ²Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, ³School of Biological, Biomedical & Environmental Sciences, University of Hull

Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological relevance.

Developmental Biology

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots

Ines Rauschert^*2, Juan Claudio Benech^*2, Martha Sainz¹, Omar Borsani¹, Mariana Sotelo-Silveira¹

¹Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República (UdelaR), ²Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)

The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit).

Developmental Biology

Polysome Purification from Soybean Symbiotic Nodules

María Martha Sainz¹, Carla Valeria Filippi¹, Guillermo Eastman^2,3, Mariana Sotelo-Silveira¹, C. Mauro Martinez¹, Omar Borsani¹, José Sotelo-Silveira^2,4

¹Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, ²Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, ³Department of Biology, University of Virginia, ⁴Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República

This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.