JoVE Logo

Sign In

University Innsbruck

1 ARTICLES PUBLISHED IN JoVE

image

Developmental Biology

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
Willi Kari 1, Fan Zeng 1, Lena Zitzelsberger 1, Johannes Will 1, Ute Rothbächer 1
1Department of Evolution and Developmental Biology, Zoological Institute, University Innsbruck

We present transient transgenesis and gene knockdown in Ciona intestinalis, a chordate sister group to vertebrates, using microinjection and electroporation techniques. Such methods facilitate functional genomics in this simple invertebrate that features rudimentary characteristics of vertebrates, including notochord and head sensory epithelia, and many orthologs of human disease associated genes.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved