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Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Reliable and High Efficiency Extraction of Kidney Immune Cells
Ravi Nistala 1, Alex Meuth 2, Cassandra Smith 3, Aroor Annayya 3
1Division of Nephrology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 2Division of Biomedical Sciences, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 3Division of Endocrinology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital

Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.

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