Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

Catherine Corbel¹, Edith Heard¹

¹Unité de Génétique et Biologie du Développement, Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934

Trophoblast giant cells (TGCs) play a key role in the placenta to ensure a healthy pregnancy. We present a protocol for assessing the transcriptional status of genes in TGCs by nascent fluorescent in situ hybridization on cryostat sections of post-implantation embryos or short-term cultures of embryonic day 7 ectoplacental cones.

Biology

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Antonia Hauth¹, Rafael Galupa¹, Nicolas Servant², Laura Villacorta¹, Kai Hauschulz³, Joke Gerarda van Bemmel⁴, Agnese Loda¹, Edith Heard^1,5

¹EMBL: European Molecular Biology Laboratory, ²Institut Curie, ³Agilent Technologies, ⁴Genmab BV, ⁵Collège de France

This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.