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IBM Research - Zurich

1 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
Aditya Kashyap 1, Julien F. Cors 1, Robert D. Lovchik 1, Govind V. Kaigala 1
1IBM Research - Zurich

We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.

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