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MedImmune

3 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Jacqueline Naylor 1, Alessandra Rossi 1, Christopher Brankin 2, David C. Hornigold 1
1Cardiovascular & Metabolic Disease, MedImmune, 2Lab Automation and Support, MedImmune

Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.

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Cancer Research

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
Michael Surace 1, Karma DaCosta 1, Anna Huntley 1, Weiguang Zhao 1, Christopher Bagnall 2, Charles Brown 1, Chichung Wang 3, Kristin Roman 3, Jennifer Cann 1, Arthur Lewis 2, Keith Steele 1, Marlon Rebelatto 1, Edwin R. Parra 4, Clifford C. Hoyt 3, Jaime Rodriguez-Canales 1
1Laboratory of Pathology, MedImmune, 2Laboratory of Pathology, MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology, University of Texas - MD Anderson Cancer Center

A detailed protocol for a six-marker multiplex immunofluorescence panel is optimized and performed, using an automated stainer for more consistent results and a shorter procedure time. This approach can be directly adapted by any laboratory for immuno-oncology studies.

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Medicine

Incorporation of a Survivable Liver Biopsy Procedure in Mice to Assess Non-alcoholic Steatohepatitis (NASH) Resolution
Stephanie Oldham 1, Christian Rivera 2, Michelle L. Boland 1, James L. Trevaskis 1
1Cardiovascular, Renal and Metabolic Diseases, MedImmune, 2Laboratory Animal Resources, MedImmune

In clinical settings, a liver biopsy is used to assess stages of non-alcoholic steatohepatitis (steatosis, inflammation, hepatocyte ballooning, and fibrosis). Here, we illustrate a survivable liver biopsy in mice which can be used for histological analyses to enable assessment of therapeutic agents in a manner more aligned with clinical trials.

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