Johann Wolfgang Goethe-University

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Sara Tognarelli^*1,2, Benedikt Jacobs^*3,4, Nina Staiger^1,2, Evelyn Ullrich^1,2

¹Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology, Johann Wolfgang Goethe-University, ²LOEWE Center for Cell and Gene Therapy, Johann Wolfgang Goethe-University, ³Institute for Cancer Research, Department of Cancer Immunology, Oslo University Hospital, Radiumhospital, ⁴The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine, University of Oslo

A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets.

Bioengineering

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Liudmila Leppik¹, Mit B. Bhavsar¹, Karla M.C. Oliveira¹, Maria Eischen-Loges¹, Sahba Mobini^1,2, John H. Barker¹

¹Frankfurt Initiative for Regenerative Medicine, Johann Wolfgang Goethe-University, ²J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida

Here we present a protocol for the construction of a cell culture chamber designed to expose cells to various types of electrical stimulation, and its use in treating mesenchymal stem cells to enhance osteogenic differentiation.

Biochemistry

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Misbha Ud Din Ahmad¹, Alexander Fish¹, Jeroen Molenaar¹, Sridhar Sreeramulu², Christian Richter², Nadide Altincekic², Harald Schwalbe², Hans Wienk¹, Anastassis Perrakis¹

¹Oncode Institute and Division of Biochemistry, the Netherlands Cancer Institute, ²Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University

Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.