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Institute of Biotechnology, Berlin University of Technology

2 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
Eva-Maria Materne *1, Ilka Maschmeyer *1,2, Alexandra K. Lorenz 1, Reyk Horland 1,2, Katharina M. S. Schimek 1, Mathias Busek 3, Frank Sonntag 3, Roland Lauster 1, Uwe Marx 1,2
1Medical Biotechnology, Technische Universität Berlin, 2TissUse GmbH, 3Fraunhofer IWS

Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.

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Cancer Research

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
Thomas Hiller 1, Viola Röhrs 1, Eva-Maria Dehne 2, Anke Wagner 1,3, Henry Fechner 1, Roland Lauster 2, Jens Kurreck 1
1Department of Applied Biochemistry, Institute of Biotechnology, Berlin University of Technology, 2Department of Medical Biotechnology, Institute of Biotechnology, Berlin University of Technology, 3Department of Bioprocess Engineering, Institute of Biotechnology, Berlin University of Technology

The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.

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