Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and FunctionYogesh Singh 1,2, Oliver A. Garden 3, Florian Lang 2, Bradley S. Cobb 1
1Department of Comparative Biomedical Sciences, The Royal Veterinary College, 2Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen, 3Department of Clinical Science and Services, The Royal Veterinary College
Many experimental systems have been utilized to understand the mechanisms regulating T cell development and function in an immune response. Here a genetic approach using retroviral transduction is described, which is economic, time efficient, and most importantly, highly informative in identifying regulatory pathways.