The Jerzy Kukuczka Academy of Physical Education

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery

Ana R. Leda¹, Levy Dygert¹, Luc Bertrand¹, Michal Toborek^1,2

¹Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, ²The Jerzy Kukuczka Academy of Physical Education

The present protocol describes a mouse microsurgery infusion technique, which effectively delivers substances directly into the brain via the internal carotid artery.

Medicine

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

Luc Bertrand^1,2, Levi Dygert², Michal Toborek^1,3

¹Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, ²Miller School of Medicine, University of Miami, ³Jerzy Kukuczka Academy of Physical Education

We describe a mouse model of stroke induced by the occlusion of the middle cerebral artery using a silicone coated suture. The protocol can be applied to induce permanent occlusion or a temporary ischemia, followed by reperfusion.

Biochemistry

A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

Nikolai Fattakhov¹, Silvia Torices¹, Sarah Becker¹, Timea Teglas¹, Oandy Naranjo¹, Michal Toborek^1,2

¹Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, ²Institute of Physiotherapy and Health Sciences, The Jerzy Kukuczka Academy of Physical Education

Here, we describe the method for establishing a triple cell culture model of the blood-brain barrier based on primary human brain microvascular endothelial cells, astrocytes, and pericytes. This multicellular model is suitable for studies of neurovascular unit dysfunction during ischemic stroke in vitro or for the screening of drug candidates.