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SOKENDAI (The Graduate University for Advanced Studies)

5 ARTICLES PUBLISHED IN JoVE

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Biochemistry

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Masatake Kanai 1, Shoji Mano 1,2, Mikio Nishimura 3
1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, 2Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology, National Institute for Basic Biology

We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.

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Neuroscience

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
Akira Muto 1, Koichi Kawakami 1
1Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies)

Here, we present a protocol to ablate a genetically labeled subpopulation of neurons by a two-photon laser from Zebrafish larvae.

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Behavior

Measuring Active and Passive Tameness Separately in Mice
Hiromichi Nagayama 1,2, Yuki Matsumoto 1,2, Akira Tanave 1, Motoko Nihei 1, Tatsuhiko Goto 3, Tsuyoshi Koide 1,2
1Mouse Genomics Resource Laboratory, National Institute of Genetics, 2Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), 3Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine

Tameness in animals includes a reduction of avoidance responses to humans (passive tameness) and an increase in active approaches to humans (active tameness). Here, we describe detailed protocols for three behavioral tests (active tameness, passive tameness, and stay-on-hand tests) to separately measure the active and passive tameness of mice.

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Neuroscience

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice
Hidenobu Mizuno 1,2,3, Shingo Nakazawa 2,3, Takuji Iwasato 2,3
1International Research Center for Medical Sciences (IRCMS), Kumamoto University, 2Division of Neurogenetics, National Institute of Genetics, 3Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies)

We present an in vivo two-photon imaging protocol for imaging the cerebral cortex of neonatal mice. This method is suitable for analyzing the developmental dynamics of cortical neurons, the molecular mechanisms that control the neuronal dynamics, and the changes in neuronal dynamics in disease models.

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Neuroscience

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
Kazuhide Asakawa 1, Hiroshi Handa 1, Koichi Kawakami 2,3
1Department of Chemical Biology, Tokyo Medical University, 2Division of Molecular and Developmental Biology, National Institute of Genetics, 3Department of Genetics, Graduate University for Advanced Studies (SOKENDAI)

We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model.

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