In Vitro Differentiation of Mature Myofibers for Live ImagingMafalda R. Pimentel 1, Sestina Falcone 2, Bruno Cadot 2, Edgar R. Gomes 1,2
1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 2Myology Research Center, UM76-INSERM U974-CNRS FRE 361, Sorbonne University, UPMC University of Paris 6
Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.