Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental ContaminantsAlice Rotini 1,2, Loredana Manfra 1,3, Filippo Spanu 2, Marco Pisapia 1, Anna Maria Cicero 1, Luciana Migliore 2
1Institute for Environmental Protection and Research (ISPRA), Rome (Italy), 2Department of Biology, Tor Vergata University, Rome (Italy), 3Department of Biology and Evolution of Marine Organisms, Stazione Zoologica, Anton Dohrn, Naples (Italy)
This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure.