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College of Veterinary Medicine, Michigan State University

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Gurpreet Kaur 1,2, Jaimo Ahn 1, Kurt D. Hankenson 1,3, Jason W. Ashley 1,4
1Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, 2Department of Biological Sciences, University of the Sciences, 3The Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 4Department of Biology, College of Science, Technology, Engineering, & Mathematics, Eastern Washington University

Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.

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Medicine

Fracture Apparatus Design and Protocol Optimization for Closed-stabilized Fractures in Rodents
Robert L Zondervan 1,2, Mitch Vorce 3, Nick Servadio 4, Kurt D. Hankenson 2
1College of Osteopathic Medicine, Michigan State University, 2Department of Orthopaedic Surgery, University of Michigan Medical School, 3Lymann Briggs College, Michigan State University, 4College of Engineering, Michigan State University

The goal of the protocol is to optimize the fracture generation parameters to yield consistent fractures. This protocol accounts for the variations in bone size and morphology that may exist between animals. Additionally, a cost-effective, adjustable fracture apparatus is described.

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