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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
María Jesús Sánchez-Calabuig 1,2, Francisco Alberto García-Vázquez 3, Ricardo Laguna-Barraza 1, Carlos Barros-García 4, Daniel García-Parraga 5, Dimitrios Rizos 1, Alfonso Gutiérrez Adan 1, José Félix Pérez-Gutíerrez 2
1Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5Veterinary Services, L'Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013

Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.

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Environment

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images
José A. Fernandez-Gallego 1,2,3, María Luisa Buchaillot 1,2, Adrian Gracia-Romero 1,2, Thomas Vatter 1,2, Omar Vergara Diaz 1,2, Nieves Aparicio Gutiérrez 4, María Teresa Nieto-Taladriz 5, Samir Kerfal 6, Maria Dolors Serret 1,2, José Luis Araus 1,2, Shawn C. Kefauver 1,2
1Plant Physiology Section, Faculty of Biology, University of Barcelona, 2Agrotecnio, 3Programa de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Ibagué, 4Instituto Tecnológico Agrario de Castilla y León (ITACyL), 5Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 6Syngenta Spain

We present a protocol for counting durum wheat and barley ears, using natural color (RGB) digital photographs taken in natural sunlight under field conditions. With minimal adjustments for camera parameters and some environmental condition limitations, the technique provides precise and consistent results across a range of growth stages.

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