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University of León

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
David G. Valcarce 1,2, Vanesa Robles 2,3
1Department of Molecular Biology, University of León, 2INDEGSAL, University of León, 3Planta de Cultivos el Bocal, Spanish Institute of Oceanography (IEO)

This protocol describes a detailed methodology for detecting H2O2 localization within Solea senegalensis spermatozoa using a sensitive fluorochrome DCFH-DA for ROS, a live mitochondria stain for mitochondria, and DAPI for nuclei visualization, respectively. The protocol is designed to be performed within 2 h with either fresh or thawed spermatozoa.

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