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Center for Neurodegenerative Science Van Andel Institute

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
Tyler Rhinesmith 1, Bryan A. Killinger 2, Akhil Sharma 3, Anna Moszczynska 3
1Physiology, Michigan State University, 2Center for Neurodegenerative Science Van Andel Institute, 3Pharmaceutical Sciences, Wayne State University

A method for stabilizing and separating native protein complexes from unmodified tissue lysate using an amine-reactive protein cross-linker coupled to a novel two-dimensional polyacrylamide gel electrophoresis (PAGE) system is presented.

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