Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister HistonesStamatios Liokatis 1
1Department of Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP-Berlin)
This protocol describes the reconstitution of nucleosomes containing differentially isotope-labeled sister histones. At the same time, asymmetrically post-translationally modified nucleosomes can be generated after using a premodified histone copy. These preparations can be readily used to study modification crosstalk mechanisms, simultaneously on both sister histones, by using high-resolution NMR spectroscopy.